Rat CCL3/MIP1a ELISA Kit
SKU: 29955986197

Rat CCL3/MIP1a ELISA Kit

$220.05 $244.50
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Description

Rat CCL3/MIP1a ELISA KitThe Rat CCL3 MIP1a ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat MIP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat MIP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those

The Rat CCL3/MIP1a ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP1a in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 29955986197

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4.6 ★★★★★
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Verified Purchase
Mike Athey
Dallas, US
★★★★★ 2
Content seems good, but support files/code are insufficient, and the code seems illogical in places
Format: Paperback
This books starts out great and the content/approach seems solid. But I ran into several issues when the coding portions were introduced, notably when it came to collisions and destroying enemy game objects. There's scripting to set up a bullet behavior, but from the outset the behavior is buggy, and when the "explosion" behavior is introduced, there's no coding to actually destroy the object you're introducing the explosion behavior to. On top of that, the explosion behavior is tied to the playerObj's "onDeath" function, so ... I'm not sure how any of that ties into destroying an enemy game object. This is about 150 pages in. The go-to method in these cases is, of course, to reference the support files, but these files only include the code and the book images. What's really needed is the Unity project files to ensure the code is being implemented in the components of the game objects and seeing the core structure there. With only the scripts, you can only check your scripts against those, but you can't be sure you're utilizing them correctly and tying it all together in Unity. Granted, I'm still learning the platform, but things like where you're expected to fire a bullet that has no "destroy gameObject" function into an enemy gameObject that also has no such behavior ... and the behavior is tied to the player? There's definitely something amiss. All the while you're dealing with "soft locks" in the test gameplay that could have been immediately addressed, but aren't. And you can't really move forward with such core inconsistencies in place because you fear the problems would just compound from there.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 31, 2023
S
Verified Purchase
Shannon
Louisville, US
★★★★★ 5
Very helpful
Format: Paperback
Given as a gift. They wanted to learn about coding and how it works. They found it very helpful and was able to understand it
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 9, 2024
C
Verified Purchase
ChargingBull
Omaha, US
★★★★★ 5
Lots of uses in Roblox
Format: Paperback
Son loves getting these for birthdays and holidays.
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Reviewed in the United States on July 6, 2025
J
Verified Purchase
jeanet
Bozeman, US
★★★★★ 5
Easy to follow
Format: Paperback
My children are able to follow along. Directions aren't too simplistic nor too complicated. Good for adults too. They are using the skills they learned and adding new ones. Happy with this purchase.
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Reviewed in the United States on May 27, 2023
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Verified Purchase
Internetaholic
Omaha, US
★★★★★ 4
Maybe Outdated
Format: Paperback
I got this for my brother who is attempting to make a game in Roblox. He said a bit of the information doesn't match the current game-making system, but that the book does have a lot of good information regardless.
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Reviewed in the United States on November 26, 2025

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