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Description
Human GLP-2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Glucagon Like Peptide 2 (GLP-2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Glucagon Like Peptide 2 (GLP-2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Glucagon Like Peptide 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Glucagon-like peptide 2 (GLP-2) is a 33-amino acid peptide. GLP-2 regulates gastrointestinal motility, gastric acid secretion, intestinal hexose transport, and enhances the barrier function of intestinal epithelial cells. It significantly increases the surface area of the mucosal epithelium by stimulating crypt cell proliferation and inhibiting apoptosis of enterocytes and crypts. In conditions of small and large bowel inflammation, GLP-2 can reduce mortality and mucosal damage. Its effects are transduced by the GLP-2 receptor, a G protein-coupled receptor expressed in enteroendocrine cells of the stomach, small intestine, and colon. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.8 ★★★★★
Based on 1766 reviews
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Product Reviews
★★★★★ 5
Good steamer and brand name.
Style: Steam Starter
I gave 4 stars as Shark Steamer mop S1000 is a good product. My last one was the purple steam mop and lasted 15 years. It was used at least once a week on all floors and for mess. It has the power steam and wipe the dirt away. Fast and easy. I have sealed hard wood floors, lots of tile, have always had two large dogs and one messy spouse who seams to drop everything on the kitchen floor, and brings in mud. (The dogs are cleaner). Needless to say, it gets good use. Fill the tank, wait as instructions say 2-3 minutes, you will hear the steam and it ready. I like the washable pads and ordered six extra to have them on had. The last was you had to fill the tank, not removable. This new one has a removable tank which I like the idea. Easy to dump out any water remain to let dry. However, maybe because it’s new, I find the tank is a bit hard to remove, the instructions say to pull up and out. You have to just pull out. The button under the tank on the machine its self does push in to allow the tank to snap back into place, but to remove it takes a little figure power to pull out. It needs a tank release button to make easier for arthritis hands. But I am still sticking with this machine and brand, hope it does me just as well as the last one.
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Reviewed in the United States on February 1, 2026
★★★★★ 4
A good beginning Steam Mop for the price
Style: Steam Starter
I have been curious about Steam Mops for awhile now and thought for the price - why not? Pros: Price and general quality of product; simplicity of the "mechanics" of a steam mop, and watching the steam actually come out of the body of the mop into the pad; I've cleaned a few pretty dirty surfaces; but no tile grout areas like some of the impressive videos; generally I suppose I have a sense that I am REALLY cleaning something cause there's steam! Maybe it's just a mind thing you know the power of suggestion. Cons: cord could be longer; and the element that you fill with water is a little sticky to actually remove; it's almost as if you feel like your going to break something by having to 'force' the water bottle away from the mop; every time I do this - I still feel I may break it. Also; the pad to clean the floor with is large with most of the steam actually exiting right in the middle - so I have found myself using a basic "floor cleaner" from a squeeze bottle in order to actually feel like I am getting my floors extra clean. I don't put anything other than water in the water-bottle for the mop - without the use of anything else; the floors do appear cleaned - and evidenced by the dirt on the pad. I might suggest purchasing a few extra pads so you can keep them clean. I toss mine in the wash - seems to work.
Conclusion: I would recommend this model mostly for those who just want to give the ole' steam mop craze a try; you can't beat the price for a basic-simple model.
I may upgrade one day but for now - this works fine.
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Reviewed in the United States on June 14, 2022
★★★★★ 5
Simple and effective.
Style: Steam Starter
I'm glad I purchased this model. I previously had a more complex model with the detachable steamer. Eventually the steamer would no longer attach to the handle, the mechanism wore out. This one is super simple. Put water in. Push down to make steam. It gives off plenty of steam. I'm confident that my floors are getting clean and there are no frills to get in the way of productivity. I will be purchasing more pads, as others have recommended.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Buy it and you’ll be happy that you did!
Style: Steam Starter
I bought this for my fifth wheel trailer and it is wonderful! I love how easy it is to use on floors. It saves me time as it dries quickly. I actually mix some floor cleaner in with the water and it leaves a nice clean smell. I can plug it in to one outlet and do the bathroom as well as the living room floors because it has a nice length to the cord. It has no leaks and is the perfect size to fit in my front closet. Best steam mop by far!
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Reviewed in the United States on April 18, 2026
★★★★★ 5
CLEAN at last!
Style: Steam Starter
This is WONDERFUL! My kitchen floor is a dark, swirly stone pattern tile and it never felt truly CLEAN before... now it does... grateful for steam!
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Reviewed in the United States on June 3, 2026